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. 2019 Nov 29;222(23):jeb207787. doi: 10.1242/jeb.207787

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© 2019. Published by The Company of Biologists Ltd

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Fig. 3. — How to calibrate a photo-ionization detector (PID) to any odorant it can detect. (A) Schematic for PID calibration. (B) Air required to completely deplete the odorant vs flow rate. (C) PID traces to complete depletion of odorant. (D) Integrated PID signal vs flow rate with spline interpolant (red). (E) Cumulative odorant vs time. Dashed line is at 1.02 mmol. (F) Mean odorant flux vs flow rate. (G) Odorant vs PID value, for ethyl acetate (red crosses), ethanol (blue circles), 2-butanone (green diamonds) and pentyl acetate (pink squares) fitted by a linear relationship. (H) Correlation coefficient (r²) of fit vs PID sensitivity. MFC, mass flow controller.