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. 2015 Jul 9;34(3):1494–1502. doi: 10.3892/or.2015.4120

Table II.

Primers for construction of STC2 promoter in pGL3 luciferase plasmid.

Primer site Sequences 5′-3′ Product (bp)
−290 to +43 F: 5′-CCGCTCGAGACTCCTTCATTCAAGTGACA-3′    334
R: 5′-CCCAAGCTTACCAAAGCCAGGGTCATG-3′
−647 to +43 F: 5′-CCGCTCGAGAACTTTCCCAACCCGATGT-3′    691
R: 5′-CCCAAGCTTACCAAAGCCAGGGTCATG-3′
−1,313 to +43 F: 5′-CCGCTCGAGAACTTTCTCCTTCCCTCCA-3′ 1,357
R: 5′-CCCAAGCTTACCAAAGCCAGGGTCATG-3′
−1,313 to −620 F: 5′-CCGCTCGAGAACTTTCTCCTTCCCTCCA-3′    694
R: 5′-CCCAAGCTTGTCACACCCACATCGGGTT-3′

STC2, stanniocalcin 2; F, forward; R, reverse.