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Identification of phosphorylated subunits by mass spectrometry.
| Total peptide matches after exposure to UV (min) | ||||
|---|---|---|---|---|
| Total peptide matches after exposure to UV (min) | ||||
| Subunit | Phospho-site | 0 | 30 | 240 |
| CSN1 | pS468 | 2 | 0 | 1 |
| pS474 | 6 | 5 | 9 | |
| pT479 | 4 | 4 | 4 | |
| pS483 | 0 | 0 | 2 | |
| pS485 | 0 | 0 | 2 | |
| CSN7 | pS232 | 1 | 0 | 2 |
CSN was purified using the Flag tag on the eCSN5 subunit before and after 30 and 240 min HeLa/eCSN5 cell irradiation with UV at 25 J/m2 followed by analysis of phosphopeptides analyzed by mass spectrometry.
