FIG. 1.

OGD/R decreases H9C2 cell viability and increases the expression of miR-17-3p. OGD/R was performed by means of free FBS and no-glucose Dulbecco's modified Eagle's medium (DMEM) in 5% CO₂, 1% O₂, and 37°C for 6 hours, then higher-glucose DMEM, 10% FBS and 1% penicillin-streptomycin in 5% CO₂, 95% air, and 37°C for 1 hour. H9C2 cells were seeded in 96-well plate at 4 × 10³ cells/hole for 24 hours. Then, H9C2 cells were treated with OGD/R for 24 hours or 48 hours. (A) Cell viability was detected by MTT assay. (B) The expression level of miR-17-3p was analyzed via qRT-PCR assays. Values were presented by mean ± SD, and the relationship between the two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, *p < 0.05, **p < 0.01). FBS, fetal bovine serum; OGD/R, oxygen-glucose deprivation/reoxygenation; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.