FIG. 2.

RS reduces OGD/R injury in H9C2 cell. H9C2 cells were seeded in 96-well plate at 4 × 10³ cells/hole. After 24 hours, the cells were treated with different concentrations of RS for 24 hours. RS + OGD/R group was performed as follows: H9C2 cells were incubated by 1 μM RS for 3 hours. Then, the cells were performed by OGD/R and normal circumstance for 21 hours after the cells were washed by PBS. (A, C) MTT assay detected cell viability. (B, E) miR-17-3p level was evaluated by qRT-PCR assays. (D) LDH leakage was assessed by colorimetric assays. (F-H) The levels of LC3II/LC3I and cleaved caspase-3 were measured by western blot. Values were presented by mean ± SD, and the relationship between two groups was analyzed by ANOVA with Tukey's test (* vs. Control group, ^ vs. OGD/R group; **^^p < 0.01). RS, rosuvastatin; LDH, lactate dehydrogenase.