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. 2019 Dec 4;21(6):323–330. doi: 10.1089/cell.2018.0053

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© Xiaoqin Wang, et al., 2019. Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 4. — Effects of miR-17-3p inhibitor combined RS on OGD/R injury. NC group: NC transfected H9C2 cell for 24 hours; miR-17-3p inhibitor group: miR-17-3p inhibitor transfected H9C2 cells for 24 hours; OGD/R + NC group: NC transfected H9C2 cell for 24 hours, meanwhile, H9C2 cells were put in OGD/R model and normal culture conditions; OGD/R + miR-17-3p inhibitor group: miR-17-3p inhibitor transfected H9C2 cells for 24 hours, meanwhile, H9C2 cells were placed in OGD/R model and normal culture conditions; RS + OGD/R + NC group: RS and NC treated H9C2 cell for 3 hours. Then, H9C2 cells-treated NC were placed in OGD/R model and normal culture conditions for 21 hours; RS + OGD/R + miR-17-3p inhibitor group: RS and miR-17-3p inhibitor treated H9C2 cell for 3 hours. Then, H9C2 cells-transfected miR-17-3p inhibitor were placed in OGD/R model and normal culture conditions for 21 hours; (A) The expression of miR-17-3p was analyzed using qRT-PCR assays. (B) Cell viability was measured with MTT assays. (C) LDH leakage was detected by colorimetric assays. (D) Cell apoptosis was evaluated using flow cytometer with dead cell apoptosis kit. (E) The levels of LC3II/LC3I, cleaved caspase-3, and Cyto c were detected by western blot. Values were presented by mean ± SD, and the relationship between the two groups was analyzed by ANOVA with Tukey's test (# vs. NC group, ^ vs. OGD/R + NC group, & vs. RS + OGD/R + NC group; ^#&p < 0.05, ^**##&&p < 0.01).