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. 2019 Dec 4;21(6):323–330. doi: 10.1089/cell.2018.0053

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© Xiaoqin Wang, et al., 2019. Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 5. — Effects of miR-17-3p mimic combined RS on OGD/R injury. NC group: NC transfected H9C2 cells for 24 hours; miR-17-3p mimic group: miR-17-3p mimic transfected H9C2 cells for 24 hours; OGD/R + NC group: NC transfected H9C2 cells for 24 hours, meanwhile, H9C2 cells were put in OGD/R model and normal culture conditions; OGD/R + miR-17-3p mimic group: miR-17-3p mimic transfected H9C2 cells for 24 hours, meanwhile, H9C2 cells were placed in OGD/R model and normal culture conditions; RS + OGD/R + NC group: RS and NC treated H9C2 cells for 3 hours. Then, H9C2 cells-treated NC were placed in OGD/R model and normal culture conditions for 21 hours; RS + OGD/R + miR-17-3p mimic group: RS and miR-17-3p mimic treated H9C2 cells for 3 hours. Then H9C2 cells-transfected miR-17-3p mimic were placed in OGD/R model and normal culture conditions for 21 hours; (A) The expression of miR-17-3p was analyzed using qRT-PCR assays. (B) Cell viability was measured with MTT assays. (C) LDH leakage was detected by colorimetric assays. (D) The levels of LC3II/LC3I, cleaved caspase-3, and Cyto c were detected by western blot. Values were presented by mean ± SD, and the relationship between two groups was analyzed by ANOVA with Tukey's test (# vs. NC group, ^ vs. OGD/R + NC group, & vs. RS + OGD/R + NC group; ^#&p < 0.05, ^**##&&p < 0.01).