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Circulating NETosis biomarkers in patients with DFU. Circulating levels of citH3 (A), cfDNA (B), and nucleosomes (C) of DFU patients were significantly higher than those of diabetic patients without foot ulcers and of the healthy controls. The results are expressed as the means ± standard deviations for each group. *p < 0.05, **p < 0.01. cfDNA, cell-free DNA; citH3, citrullinated histone 3; CTRL, control; DFU, diabetic foot ulcer; DM, diabetes mellitus.
