Fig.5.
MSC accelerated microglial clearance of Aβ in vitro. a) MG6 cells were co-cultured with MG6 cells or MSC for 24 h. Amount of Aβ1–42 in MG6 cells was measured before exposure to Aβ [Aβ (–)], after exposure to Aβ for 3 h [Aβ(+)], and 3 h after washout of Aβ (Degradation), using ELISA. MG6 cells were exposed to fluorescent labeled Aβ and MG132 for 15 min. b) Representative images of labeled Aβ taken up by MG6 cells. Scale bar; 20 μm. c–g) Fluorescent intensity of labeled Aβ was analyzed by flowcytometry as Aβ uptake. d) Uptake of monomeric Aβ (HFIP/DMSO), oligomeric Aβ (4°C 24 h) and fibrillar Aβ (37°C 24 h). e) Uptake of Aβ when secretion from MSC was blocked by Golgistop. f) Effect of conditioned medium of MG6 cells (Con) or conditioned medium of MSC (CM) on Aβ uptake by MG6 cells. Conditioned medium was filtered through 100 kDa ultrafiltration filter (100 kDa), 10 kDa ultrafiltration filter (10 kDa), or non-filtered [Filter (–)]. g) Effect of heated conditioned medium (Heat). N.D., not detected; n.s., not significant.