Skip to main content
. 2019 Dec 12;25(23-24):1658–1666. doi: 10.1089/ten.tea.2019.0031

FIG. 2.

FIG. 2.

Purity and MSC marker expression in MACS-defined CD34+ adventitial cells. Adventitial cells are isolated after CD31 and CD45 depletion, by incubation with anti-CD34 microbeads and capture of magnetically labeled CD34+ cells. (A–C) Flow cytometry analysis of freshly derived adventitial cells. (A) Virtually all cells are CD34+CD146− (98.7%), while a subset is CD34+CD146+ (1.16%). (B) Virtually all cells are CD34+CD31− (99.27%), while a subset is CD34+CD31+ (0.26%). (C) The majority of cells are CD34+CD45− (90.45%), while a subset of cells is CD34+CD45+ (9.38%). (D–G) Canonical MSC marker expression among freshly isolated and MACS-defined CD34+CD146−CD31−CD45− adventitial cells. Canonical MSC markers are expressed at high frequency in MACS-defined adventitial cells, including (D) CD44 (98.56%), (E) CD73 (85.91%), (F) CD90 (99.55%), and (G) CD105 (98.10%). Data shown as overlaid histograms with stained MACS-defined adventitial progenitor cells in red in comparison to unstained controls in black. Representative data shown, repeated in biologic triplicate. MSC, mesenchymal stem/stromal cell. Color images are available online.