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. 2019 Dec 16;2(6):434–440. doi: 10.1089/crispr.2019.0048

FIG. 2.

FIG. 2.

The type IV-A1 CRISPR-Cas variant from P. aeruginosa PA83 mediates RNA-guided interference in vivo. (A) Plasmid transformation efficiency assay described in the methods section. Small arrows on plasmids indicate a T7 promoter. (B) A reduction in plasmid transformation efficiency was observed when cells harboring a CRISPR array with TS1, csf1, csf2, csf5/cas6, csf3, and csf4/dinG were transformed with the target compared to those transformed with the non-target plasmid. (C) Plasmid maintenance assay described in the Methods section. Small arrows on plasmids indicate a T7 promoter. (D) An interference defect is observed when any component is removed and when the Csf4/DinG DEAH-box is mutated (D336A/E337A). TS1 was used in each deletion line and the Csf4/DinG mutant. As each condition represents a different competent cell line, an uninduced control was always included (Supplementary Fig. S2). TS, targeting spacer; NTS, non-targeting spacer.