Figure 1.

Overview of Fast-Seq, a Tn5-based packaged ssAAV genome sequencing method. Preparations of rAAV undergo ssDNA extraction, second-strand synthesis to generate dsDNA, tagmentation with adapter-loaded Tn5 transposomes, indexing, QC validation, short-read sequencing on Illumina MiSeq, mapping reads to the transfer vector plasmid used to generate the input rAAV, and sequence validation of the packaged genome. An example packaged genome mutation (*) is shown at position 7. dsAAV, double-stranded AAV; dsDNA, double-stranded DNA; rAAV, recombinant AAV; ssAAV, single-stranded adeno-associated virus; Tn5, transposase.