Figure 4.

Dilution series validates input requirements for sequencing ssAAV genomes and ITRs with Fast-Seq. (a) NGS sequence validation of recombinant packaged ssAAV genomes depicting the expected sequencing depth and coverage from a standard MiSeq run after generating libraries with Fast-Seq. As shown, the coverage distribution will vary along the length of the genome due to bias in Tn5 fragmentation. (b) Zoom-in on the depth and coverage of the corresponding ITRs from (a). (c) Table highlighting the relevant metrics from the dilution series from (a) and (b). Avg, average; rep, replicate; Vg, vector genomes.