Reagent	1 sample (μL)
5 × Tn5 reaction buffer	0.64
DNase/RNase-free water	1.2
100% DMF*	0.16
Total volume	2

^*Caution: DMF is a volatile carcinogen and should be handled with appropriate safety precautions in a chemical fume hood. When handling DMF, wear appropriate PPE, including a lab coat, goggles, closed toe shoes and double gloves. Dispose of any pipette tips that contact DMF in a labeled solid waste container. Clean pipettes after use.

PPE, personal protective equipment.