| Reagent | [Stock] | [Final] | Volume needed (μL) |
|---|---|---|---|
| 10 × buffer | 10 × | 1 × | 20 |
| Endonuclease | 1 U/μL | 10 U | 10 |
| Exonuclease | 10 U/μL | 40 U | 4 |
| ATP | 1 mM | 25 mM | 8 |
| ssAAV 1E11 full particles | — | 1E11 | X |
| DNase/RNase-free water | — | — | X |
| Total volume | — | — | 200 |
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