Reagent	[Stock]	[Final]	Volume needed (μL)
Random primer mix	60 μM	6 μM	5
dNTPs	10 mm each	2 mM	10
Klenow	5 U/μL	10 U	2
10 × NEB2 buffer	10 ×	1 ×	5
gDNA from Step 5	—	—	28
Total volume	—	—	50