FIG. 7.

The use of magnetic rods in fibrin hydrogel to help guide regeneration that can be aligned upon exposure to magnetic field. (A). An illustration showing the method of preparation of injectable hybrid hydrogel. A unidirectional structure made of aligned rod-shaped, magnetoceptive microgels within the fibrin hydrogel is generated in situ by exposure to magnetic field. Following this, the surrounding liquid prehydrogel is crosslinked, so that microgel orientation is fixed to guide aligned cell ingrowth. Adapted from Rose et al.⁵⁸ (B) Premixed fibroblasts can be seen to extend along the microgel axis (green), as visualized by stretched F-actin filaments (stained in red). Reproduced with permission Rose et al.⁵⁸ Color images are available online.