Figure 4.

Vectofusin-1 enhances transduction of TCR-stimulated primary T cells with RV. Primary human T cells were activated with TransAct T Cell Reagent in TexMACS medium supplemented with IL-2. Two days after activation, T cells were transduced with RV encoding GFP, pseudotyped with GALV (MOI 1; A) and RD114 (MOI 2; B); n = 6. Cultures were washed 6 or 24 h after transduction and analyzed on day 7 of cultivation via flow cytometry. Transduction efficiencies could be increased by adding Vectofusin-1 (10 μg/mL). Highest transduction efficiencies were obtained using a spinoculation protocol (2 h centrifugation at 400 g, 32°C). A one-way ANOVA was performed to determine significance. **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant. GALV, gibbon ape leukemia virus; RV, gammaretroviral vectors.