Figure 2.

Hybrid triple reporter mouse model to assess transduction. Mice endogenously express the membrane targeted red fluorescent protein mTomato (mT). Any cell transduced by a Cre recombinase-expressing vector will stop expressing mTomato and begin to express mG and Luciferase. This hybrid mouse is useful for in vivo luminescent imaging and fluorescent microscopy to assess cell-by-cell transduction. mG, membrane targeted GFP.