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. 2019 Dec 16;30(12):1559–1571. doi: 10.1089/hum.2019.127

Figure 3.

Figure 3.

IV injection mediates liver transduction. (A) Mice were administered IV injections of PBS (n = 1), 1e12 GC AAVrh10-Cre (n = 2), or 1e11 VP Ad5-Cre (n = 2) and imaged on day 3. Ad5-Cre mediated strong visible liver transduction. (B) Kidneys were removed from the euthanized mice and imaged. No transduction was observed except by AAVrh10-Cre in the capsule of the kidney. (C) Luciferase assay shows low levels of transduction in kidneys after IV injection. Kidneys from mice shown in Panel A were extracted and homogenized. The homogenate was used in a luciferase plate reader assay. No kidney homogenate showed higher levels than background (PBS). Luminescence is quantified in RLU. GC, genome copies; PBS, phosphate-buffered saline; RLU, relative light units.