Figure 2.

Western blot analysis of AAV2 vectors. About 1.42 × 10¹⁰ vgs of AAV2 and AAV2 K105Q vectors were resolved by denaturing SDS-PAGE. The level of SUMO-1 protein on vector capsids (a) were quantified (c) as described in the Materials and Methods section. Anti-AAV capsid B1 antibody was used as a loading control (b). The field of view pertaining to loaded samples from the entire gel is shown in the image. Exposure time for SUMO-1 and B1 antibody immune-reactive blots was 2 min 49 s and 36 s, respectively. Data presented are representative set from three independent biological replicates. Paired t-test was performed to determine the statistical significance. Data are expressed as mean ± SD, n = 3, ***p ≤ 0.001. SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SUMO, small ubiquitin-like modifier; vgs, vector genomes. Color images are available online.