Figure 5.

Ocular gene transfer in C57BL6/J mice with SUMOylation site–modified K105Q vector. A fundus imaging of murine eyes was performed 4 weeks after administration of AAV2 wild-type and AAV2 K105Q vectors. For these experiments, we used a Micron IV imaging system (Phoenix Research Laboratories, Pleasanton, CA) that employs a standard mouse objective and a field of view at 50° (1.8 mm diameter). Intensity was set at maximum and gain was set at 15 db; the frame rate was set at 6 fps for imaging of all the groups. Image analysis was performed by using Concentric Circle Plugin in the ImageJ software, and AAV2 K105Q group had a 1.57-fold higher EGFP expression in comparison to AAV2 wild-type eyes (n = 4 to 7 eyes). Representative images are shown. Color images are available online.