Activation of yki induces Homothorax (Hth) and Teashirt (Tsh) expression. Eye imaginal discs stained for (A,C,E,G,G′) Hth (green) and (B,D,F,H,H′) Tsh (green) are shown from (A,B) wild-type, (C,D) bi>yki3SA, (E,F) dpp>yki3SA and (G-H‴) hsFLP; Act>y+>Gal4 UASyki3SA (hsFLP>yki3SA) larvae. (A) Normally, Hth and (B) Tsh expression is restricted anterior to the MF in the eye. Both (C,E,G,G″) Hth and (D,F,H,H″) Tsh are upregulated, and (C-H,G‴,H‴) eye differentiation (Elav, red) is suppressed when yki3SA is overexpressed under (C,D) bi-Gal4, (E,F) dpp-Gal4 or in ‘FLP-out’ clones using (G,H) hsFLP>yki3SA. (G-H′) ‘Flp-out’ clones of yki3SA are marked by GFP (blue).