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. 2019 Dec 10;98(49):e18073. doi: 10.1097/MD.0000000000018073

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Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0

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SDS-PAGE of purified recombinant wild and mutant proteins expression. Lanes: 1, Standard protein marker; 2, induced pET-32a-PstS1-H37Rv; 3, induced pET-32a-PstS1-FJ05132; 4, induced pET-32a-PstS1-JL06035; 5, induced pET-32a-MPT64-H37Rv; 6, induced pET-32a-MPT64-FJ05395. SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis.