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. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Nat Struct Mol Biol. 2019 Jun 3;26(6):501–509. doi: 10.1038/s41594-019-0236-8

Fig. 1 ∣. Structural analysis of PF846 stalled RNCs.

Fig. 1 ∣

a, Schematic representation of the DNA constructs used to prepare PF846 stalled RNCs. b, Protein sequences from CDH1, PCSK9, and USO1 tested for stalling. The arrow indicates the main stalling site predicted from ribosome profiling data 10. c, Luciferase reporter assays using the chimeric PCSK9 nascent chain in the absence (white bar) or presence (grey bar) of PF846 (Data represent mean ± s.d., n = 3 independent experiments). d, Cryo-EM structure of the stalled CDH1-RNC in the rotated state showing A/P tRNA (green), P/E tRNA (magenta), CDH1 nascent chain (purple) and PF846 (dark green) with small and large subunit colored in cyan and grey, respectively. e, Structure of the stalled CDH1-RNC in the rotated state with A/A tRNA (orange), and P/E tRNA (magenta). Source data for panel c are available in Supplementary Data Set 3.