Skip to main content
. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Nat Struct Mol Biol. 2019 Jun 3;26(6):501–509. doi: 10.1038/s41594-019-0236-8

Fig. 6 ∣. Model of the mechanism of PF846-induced stalling.

Fig. 6 ∣

PF846 binding to the exit tunnel stalls the elongation of the nascent chains in the rotated state, with either A/A NC-tRNA or A/P NC-tRNA. Poor positioning of the 3’-CCA end prevents eEF2-GTP from catalyzing the translocation of hybrid A/P and P/E tRNAs into the P/P tRNA and E/E tRNA sites. PF846 can also stabilize the resulting non-rotated ribosome with an empty A site that may signal quality control (QC) pathways.