Table 4 ∣.
Cryo-EM data collection, refinement and validation statistics of USO1-RNC
| USO1-RNC_AP tRNA (EMD-0526, PDB 6OLI) |
|
|---|---|
| Data collection and processing | |
| Magnification | 43,478 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 55 |
| Defocus range (μm) | 1.5 to 2.5 |
| Pixel size (Å) | 1.15 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 79,322 |
| Final particle images (no.) | 38,314 |
| Map resolution (Å) | 3.5 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.8 to 8 |
| Refinement | |
| Initial model used (PDB code) | |
| 40S subunit | 6ek0 |
| 60S subunit | 5aj0 |
| tRNA | 3j7r |
| Model resolution (Å) | 3.1 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | 2.5 to 7.5 |
| Map sharpening B factor (Å2) | −91 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.117 |
| Validation | |
| MolProbity score | 1.98 |
| Clashscore | 5.36 |
| Poor rotamers (%) | 1.48 |
| Ramachandran plot | |
| Favored (%) | 89.71 |
| Allowed (%) | 9.95 |
| Disallowed (%) | 0.35 |