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. 2019 Dec 18;14(12):e0225822. doi: 10.1371/journal.pone.0225822

Fig 1. Phylogenetic analysis of newly reported Theileria spp. and other piroplasms based on the 18S rRNA locus.

Fig 1

(A) Phylogenetic relationships among piroplasm 18S rDNA sequences (~ 1.3 kb) were inferred using the maximum likelihood method. (B) Maximum likelihood analysis of piroplasms based on a 800bp alignment to illustrate the position of newly reported organisms in the context of all known Theileria spp. recorded in Australia to date. Names in bold represent sequences retrieved from GenBank that were not included in the main tree (Fig 1, A) due to their shorter length. GenBank accession codes are shown in parentheses. Bootstrap values (>60%) based on 500 replicates are indicated at the left of each node. * indicates shorter sequences (~ 800 bp) obtained in this study using a nested PCR as described by Jefferies et. al. (2007). All other new sequences (~1.3 kb) were obtained using primers Nbab_1F and 18S ApiR (Greay et al., 2018).