Fig 3.

(A) Relative abundance of colonic bacteria at the phylum level of male and female CD-1 mice collapsed by sex and age. CO: CD-1 mice fed a “Western-style” control fat. FO: CD-1 mice fed CO supplemented with 30% fish oil. BO: CD-1 mice fed CO supplemented with 30% dairy fat. EO: CD-1 mice fed CO supplemented with 30% echium oil. Abundance by counts of Bacteroidetes was greater in EO-fed mice compared to all other diet groups (P < 0.05). (B) Relative abundance of colonic bacteria at the phylum level of male and female CD-1 mice collapsed by diet group and age. Abundance by counts of Bacteroidetes was greater in females compared to males (P < 0.05). (C) Ratio of Firmicutes to Bacteroidetes of male and female CD-1 mice collapsed by diet group and age. (d) Shifts in relative abundance of the colonic Bacteroidetes of BO-fed male and female CD-1 mice at 10.5 and 13.5 months of age. Shifts in relative abundance of the colonic Bacteroidetes of EO-fed male and female CD-1 mice at 10.5 and 13.5 months of age. In BO-fed males, abundance by counts of Bacteroidetes was greater at 13.5 months of age compared to 10.5 months of age (P < 0.01). In EO-fed males, abundance by counts of Bacteroidetes was lower at 13.5 months of age compared to 10.5 months of age (P < 0.01). Values are expressed as mean ± standard error of the mean. *P < 0.05; **P < 0.01.