Fig 4. IL-22-mediated signaling enhances the gene expression of anti-apoptotic and antimicrobial proteins in lung epithelial cells.

One month after the induction of diabetes, T2DM mice were infected with 50–100 CFU of aerosolized Mtb. The mice were then treated intravenously with either recombinant IL-22 (100 ng/kg body weight, twice weekly, starting 5 months p.i.) or PBS. One month after recombinant IL-22 or PBS treatment, lung epithelial cells were isolated by immunomagnetic labeling, and mRNA expression of (A) IL-22 signaling pathway genes and (B) antimicrobial genes were determined by real-time PCR. Five mice per group were used. The mean values, SDs and p-values are shown.