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. 2019 Dec 18;14(12):e0226302. doi: 10.1371/journal.pone.0226302

Fig 5. Viability of primary endothelial cells after cryopreservation.

Fig 5

(a) Primary endothelial cells (ECs) were concentrated at 1 million cells/mL (black bars) or 2 million cells/mL (grey bars) in either ECs culture medium supplemented with 10% DMSO (CryoMedium) or foetal bovine serum supplemented with 10% DMSO (CryoFBS). Cells were counted after Trypan blue staining, prior to cryopreservation and after thawing. Results are presented as the percentage of the total number of living cells after thawing on the total number of living cells prior to cryopreservation. Data represent the mean values ± SD (n = 4). ANOVA with Tukey’s test: ns: non-significant, * P-value < 0.05. (b) Transendothelial electrical resistance (TEER) of non-cryopreserved P1 ECs (Fresh, white bar) and P1 ECs post-cryopreservation (CryoFBS, grey bar) 9 days after seeding. Data represent the mean values ± SD (n = 8). (c) Permeability values of 4 kDa and 150 kDa FITC-conjugated dextrans through non-cryopreserved P1 ECs (Fresh, white bar) and P1 ECs post-cryopreservation (CryoFBS, grey bar) 9 days after seeding. Data represent the mean values ± SD (n = 4). T-test: ns: non-significant (P-value > 0.05).