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. 2019 Dec 6;15(12):e1008491. doi: 10.1371/journal.pgen.1008491

Fig 4. TGF-β signalling is required in MB progenitors to control EcR-B1 expression during consolidation of α’β’ MB fate.

Fig 4

(A-C) Adult MB lobes from control (A), OK107>babo RNAi asense>GAL80 (B), and OK107>EcR-DN asense>GAL80 (C) brains stained with anti-FasII antibody (magenta). Green: GMR26E01-LexA-α’β’-MB-driven mGFP in A-C. (D) Quantification of the number of adult α’β’ MB neuron cell bodies from control, OK107>babo RNAi asense>GAL80 and OK107>EcR-DN asense>GAL80 brains. Statistical comparison to the control: N.S. Not Significant; ***, p<0.001 (two tailed t test). (E-G) Adult MB lobes from control (E), worniu>babo RNAi (F), and worniu>EcR-DN (G) brains stained with anti-FasII antibody (magenta). Green: GMR26E01-LexA-α’β’-MB-driven mGFP in E-G. (H) Quantification of number of adult α’β’ MB neuron cell bodies from control, worniu>babo RNAi and worniu>EcR-DN brains. Statistical comparison to the control: N.S. Not Significant; ***, p<0.001 (two tailed t test). (I-J’) WL3 MB cell bodies from control (I,I’) and OK107>babo RNAi asense>GAL80 (J, J’) brains stained with anti-EcR-B1 antibody (magenta). Green: GAL4-OK107-driven mGFP in I-J’. (L-M) WL3 MB cell bodies from control (L) and worniu>babo RNAi (M) brains stained with anti-EcR-B1 antibody (magenta). Anti-N-cadherin (green) staining was used as landmark in locating MB neurons. Cx, MB Calyx. NB, neuroblast; G, ganglion mother cell; N, neurons.