Table 3.

Oxidation of rac‐sec‐allylic alcohols 1 a–2 a employing HMFO variants in the presence of air, 2 and 4 bar O₂ pressure.^[a]

Entry	Substr.	Variant	Conv. [%]
			air	O2 (2 bar)	O2 (4 bar)
1	1 a	wt	16	13	10
2	1 a	V465T	50	31	26
3	1 a	V465 S	50	35	26
4	1 a	V465T/W466H	25	18	9
5	1 a	V367R/W466F	18	9	7
6	2 a	wt	29	33	35
7	2 a	V465T	50	48	45
8	2 a	V465 S	50	48	44
9	2 a	V465T/W466H	50	48	46
10	2 a	V367R/ W466F	33	34	46

^[a] Condition: KPi‐buffer (200 mM, pH 7.0) containing the oxidases (14.2 μM final concentration in 500 μL reaction volume in 4 mL glass vials), catalase from Micrococcus lysodeikticus (15 μL, 170000 U/mL), 1 a–2 a (50 mM). The reaction mixtures were shaken for 16 hours (170 rpm, 21 °C) and extracted with ethyl acetate (2×300 μL), dried with Na₂SO₄ and analyzed by GC‐FID. Conversions were measured based on area ratio of ketone to substrate. Reactions were done in duplicate.