Table 4.

Enantioselectivity of oxidation of substrate 4 a using HMFO variants at ambient air pressure and at 1.5 bar O₂.^[a]

Entry	Variant	O2 [bar]	Conv. [%]	ees [%][b]	E
1	V465S	1.5	58	62	5
2	V465S	ambient	55	95	29
3	V465T	1.5	54	73	9
4	V465T	ambient	50	73	14

^[a] Condition: KPi‐buffer (200 mM, pH 7.0) containing the oxidases (2.1 μM final concentration in 1 mL reaction volume in 4 mL glass vials), catalase from Micrococcus lysodeikticus (30 μL, 170000 U/mL), the substrate (50 mM), 10% v/v glycerol as cosolvent. The reaction mixtures were shaken for 16 hours (170 rpm, 21 °C; additional 1.5 bar O₂ for the mixtures with O₂ pressure) and extracted with ethyl acetate (2×500 μL), dried with Na₂SO₄ and analyzed by GC‐FID and HPLC.

^[b] Ee_s values were measured by using HPLC equipped with chiral column.