Table 6.

Semi‐preparative scale oxidation using HMFO V465S.^[a]

Entry	Substr.	Conv. [%]	Isolated yields b [%]	ee s [%]	E
1	1 a	50	70[b]	>99	>200
2	2 a	53	33	92	32
3	3 a	35	54	35	7
4	4 a	52	64	92	40
5	5 a	31	31	35	11

^[a] Condition: KPi‐buffer (200 mM, pH 7.0) containing the oxidase (14.2 μM final concentration in 25 mL reaction volume), catalase from Micrococcus lysodeikticus (750 μL, 170000 U/mL) and the substrate (50 mM). The reaction mixtures were shaken for 48 h (170 rpm, 21 °C) and extracted with ethyl acetate (2×50 mL), dried with Na₂SO₄ and analyzed by GC‐MS. Conversions were measured based on area ratio of ketone to substrate. The percentage of isolated yield refers to the conversion achieved.

^[b] The remaining substrate was isolated in quantitative yield with respect to the observed conversion.