Figure 1. Design, synthesis and fluorogenicity of Gemini-561.
(a) Synthesis of Gemini-561. (b) Concept of fluorogenic response of Gemini-561 to environment (organic solvent) and aptamer. (c) Absorption and excitation spectra of Gemini-561 (200 nM) in water and methanol and SRB (200 nM) in water. Results were found identical in n = 3 independent experiments. (d) Fluorescence spectra of Gemini-561(200 nM) in water and methanol and SRB (200 nM) in water. Results were found similar in n = 3 independent experiments.