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. Author manuscript; available in PMC: 2020 Apr 21.
Published in final edited form as: Nat Chem Biol. 2019 Oct 21;16(1):69–76. doi: 10.1038/s41589-019-0381-8

Table 1. Spectral and biochemical properties of Gemini-561 alone or in complex with SRB-2 or o-Coral aptamers.

λAbs max
(nm)		 ε
(M-1.cm-1)		 λEm max
(nm)		 Q.Y. Fluorescence Enhancement KD
(nM)		 Relative Brightness
eGFP 490   39,200 508 0.68 / / 1.00
Buffer 535 100,000 590 0.02   1.0 / 0.08
MeOH 561 162,000 580 0.31 10.3 / 1.88
SRB-2 567   98,000 597 0.04   1.2 441 ± 167.5 0.15
o-Coral 580 141,000 596 0.58 12.8 73 ± 1.5 3.07

Measures were performed in selection buffer (40 mM phosphate buffer pH7.5, 100 mM KCl, 1mM MgCl2 and 0.05% Tween-20). Fluorescence quantum yield (QY) and enhancement were obtained at an excitation wavelength of 530 nm. KD values are the mean of n = 3 independent experiments ± 1 SD.