Skip to main content
. Author manuscript; available in PMC: 2019 Dec 18.
Published in final edited form as: Anal Chem. 1990 Sep 15;62(18):2005–2012. doi: 10.1021/ac00217a020

Table I.

Decay Times and Amplitudes for ACF, AFA, and AO, and for Two-Component Mixtures of ACF, AFA, and AO

τ1, ns F1 χR2
sample expecteda found expectedb found FD PM Spec
ACF 3.95 3.98 1.0 1.0 0.3 0.6
AFA 11.72 12.04 1.0 1.0 0.5 0.8
AO 3.69c 3.73 1.0 1.0 0.1 1.2
ACF & AFAe 8.98d 8.47 1.0 1.0 46.7 79.3
3.95 3.79 0.32 0.31 0.5
11.72 11.19 0.68 0.69
AO & AFAf 7.29c,d 7.34 1.0 1.0 23.1 29.9
3.69 3.90 0.41 0.38 1.6
11.72 12.35 0.59 0.62
a

The lifetime values are the results from the usual FD measurements, where the emission is observed through a band-pass filter (Corning 3–71).

b

The fractional intensity values are the results from steady-state measurements.

c

The FD measurements were performed with an emission wavelength of 522 nm.

d

The results of the forced single exponential fits are not necessarily the same for the FD and PM Spec data.

e

[ACF] = 5 × 10−7 M and [AFA] = 2 × 10−5 M.

f

[AO] = 1.25 × 10−6 M and [AFA] = 2 × 10−5 M.