Fig. 1.

Migration speed of (A) and distance moved by (B) 4T1 cells treated with varying doses of DETA/NO using in vitro migration assay. C. Number of metastatic lesions in the lungs of tumor bearing, WT and Nos2^−/− mice that are untreated or treated with 30 mg/L indomethacin (n≥6). D. Tumor volume measurements in WT and Nos2^−/− mice that are untreated or treated with 30 mg/L indomethacin (non-linear regression) (n≥6). The untreated tumor bearing mice were euthanized on day 32 when the mice reached tumor limit (2000 mm³). The indomethacin treated mice were subjected to staggered euthanasia as the tumors reached the permitted size limit of 2000 mm³ and tumors and lungs were harvested. E. 4T1 viability (trypan blue assay) in presence of varying concentrations of Indomethacin. F. Survival analysis of indomethacin treated, tumor-bearing WT and Nos2−/− mice, (n = 6). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)