Fig. 3.

A. Nos2-derived high NO flux induces ‘M1’ morphology. Light microscopy images of unstimulated and 42h stimulated WT and Nos2^−/− macrophages. Differences in morphology between ‘M0’ and ‘M1’ macrophages computed using an automated algorithm to measure the flattening of BMDMs upon ‘M1’ stimulation. White arrows indicate the cells showing flattened morphology. B. Pearson correlation analysis of flattening (Sphericity^-1) and nitrite production by stimulated macrophages shows a significant correlation between reduction in sphericity/increased flattening and increasing nitrite levels. Stimulated Nos2^−/− BMDMs do not show this increase in flattening.