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Nos2 and Cox2 affect a mutual, reciprocal, paracrine regulation in vivo. A. Nos2 expression in 24h cytokines/LPS stimulated 4T1 cells. B. In vitro nitrosative capacity of 4T1 cells stimulated for 24h with cytokines/LPS. C. Nos2 and Cox2 expression observed in separate cells in 4T1 tumors. Single channel grayscale images are shown. Scale bar = 20 μm. D. Statistical analysis (Pearson correlation) of Nos2+ and Cox2+ cells based on fluorescence signal intensity.
