IL-35 and IL-10 similarly and partially reverse the modulation effects of LPC on global gene expressions in HAECs. HAECs were treated with LPC (10 μM), with or without IL-35/IL-10 (10 ng/mL) for 18 h, RNA sequencing (RNA-Seq) experiments were performed. A. Principal component analyses (PCA) of the RNA-Seq data. B. Cluster analysis. C. Summary of the number of changed genes (by 1.4-fold) by LPC, IL-35, and IL-10. D to F. Volcano plots showing the genes that are changed by LPC (D), IL-35 (E), and IL-10 (F). G. Principle of the fold change by fold change (FC/FC) plot. H & I. FC/FC plot showing the relationship between LPC-induced genes and the genes that are regulated by IL-35 or IL-10.