Fig. 4.

IL-35 is more effective than IL-10 in blocking LPC-induced innate immune signature genes in HAECs, while neither IL-35 nor IL-10 could block LPC-induced reprogramming of key immunometabolic pathways in HAECs, such as cholesterol homeostasis, biosynthesis of unsaturated fatty acids, tyrosine metabolism, glycolysis, and pyruvate metabolism. HAECs were treated with LPC (10 μM), with or without IL-10 (10 ng/mL) for 18 h, RNA sequencing (RNA-Seq) experiments were performed. A. Venn diagram of the Gene set enrichment analysis (GSEA) pathways that are upregulated by LPC, downregulated by IL-35, and downregulated by IL-10 in HAECs. B. Representative GSEA plot from the downregulated pathways by IL-10. C. Representative cytokines, adhesion molecules, innate, and adaptive gene molecules that are decreased by IL-10. NES, normalized enrichment score.