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. 2019 Dec 12;7:333. doi: 10.3389/fcell.2019.00333

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Copyright © 2019 Zhuang, Xiang, Wen, Shen, Zhao, Zheng, Han, Qu, Lu and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of slc7a14 led to increased apoptosis in the zebrafish retina. TUNEL assay was used to detect apoptosis in larval retinas at 3 and 5 dpf. (A,B) Slc7a14 knockdown resulted in significant increase of TUNEL + cells in the retina including GCL, INL, ONL and RPE at 3 dpf (A) and 5 dpf (B). Scale bars = 50 μm. (C,D) Statistical results for the numbers of TUNEL positive cell at 3 dpf (n = 6 for each group) and at 5 dpf (n = 3 for each group). Bar plots were shown as the mean ± s.e.m. T-test was performed between the two groups. ^∗P < 0.05, ^∗∗∗P < 0.001.