FIGURE 2.

Chilodontopsis depressa morphology and draft genome profile. (A) Cladogram showing the evolutionary relationships within the Synhymeniida, Nassophorea, and Phyllopharyngea based on morphological data. Parts (a–d) are original and part (e) is from Jankowski (1973). 1, Synhymenium restricted on left ventral sometimes dorsal surface; 1′, synhymenium extends from left to right ventral surface; 1^″, synhymenium absent; 2, synhymenium usually comprises three adoral polykinetids; 2′, synhymenium comprises more than three adoral polykinetids; 3, free-living, merotelokinetal division, oral kinetofragments present; 3′, sessile, division by budding, oral kinetofragments absent. (B) Bar chart showing the distribution of intron sizes calculated by customized Perl script. Pink bars represent percentage of introns frequency. Introns over 200 bp in length were integrated in the right bar. (C) Combining genome and transcriptome sequencing identified 5′-GT-AG-3′ as the representative motif with the third conserved A site by WebLogo3. (D) Pie chart showing the proportions of different types of non-coding RNA predicted and calculated by RepeatMask annotation: tRNA (purple), snRNA (orange), rRNA (brown), and other kind (yellow). (E) The whole genome was annotated using Interproscan and Gene Ontology (GO) enrichment analysis was performed by WEGO of C. depressa (blue) and T. thermophila (green). Bar chart showing the percentages of gene numbers.