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. 2019 Jul 5;18(1):129–140. doi: 10.1111/pbi.13178

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© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Analysis of T‐DNA integration site in GLRW‐3. The T‐DNA was inserted in an intergenic region on chromosome 1D. The genes upstream (TraesCS1D02G296900.1 and TraesCS1D02G297000.1) and downstream (TraesCS1D02G297100.1 and TraesCS1D02G297200.1) of the T‐DNA insertion site are shown with coordinates based on the annotation deposited in Ensembl Plants (http://plants.ensembl.org/Triticum_aestivum/Info/Index). Various changes occurred to the T‐DNA or wheat chromosomal DNA are summarized in the box at the bottom.