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. 2019 Jun 20;18(1):155–171. doi: 10.1111/pbi.13183

Figure 2.

Figure 2

Ectopic expression of GmWRI1s in soybean hairy roots. Soybean hairy roots overexpressing GmWRI1a, GmWRI1b or GmWRI1b’ were analysed for TAG production, with GFP‐expressing hairy roots as a control. Total neutral lipids were extracted and separated by thin‐layer chromatography (TLC) for GC analysis of FA contents and compositions. (a) qRT‐PCR verification of GmWRI1s expression in soybean transgenic hairy root lines. (b) TLC analysis of neutral lipids extracted from transgenic hairy root lines. (c) Total TAG contents in hairy root lines overexpressing GmWRI1a, GmWRI1b, GmWRI1b’ or GFP. (d) TAG FA compositions in hairy root lines. (e) Total soluble sugars in transgenic hairy root lines. (f) Sugar compositions from hairy roots expressing GmWRI1s and GFP (control). Three representative lines from more than 10 transgenic hairy root lines were analysed. All data are expressed as means ± SD (n > 4). *P < 0.05 and **P < 0.01 by Student’s t‐test for significant difference.