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. 2019 Jun 20;18(1):155–171. doi: 10.1111/pbi.13183

Figure 4.

Figure 4

Binding and transactivation of the promoters of target genes by GmWRI1s. (a–i) Subcellular localization of GmWRI1s. The fluorescence (a), bright field (b) and merge (c) images of GmWRI1a‐GFP; the fluorescence (d), bright field (e) and merge (f) images of GmWRI1b‐GFP; the fluorescence (g), bright field (h) and merge (i) images of GmWRI1b’‐GFP. Bars = 30 μm. (j) AW boxes present in genes involved in glycolysis and FA synthesis in plastids. The numbers indicate the positions of the first nucleotide C in the AW box, taking the first base of the most abundant transcription start site (TSS) as +1. Arrows indicate the complementary sequences. (k) Binding of GmWRI1s to the target gene promoters in yeast one‐hybrid assays. The transformants harbouring the empty pAbAi and pGADT7‐GmWRI1 were used as a negative control. (l) Constructs for effecter expression and promoter‐driven reporter gene system for transactivation assays. (m) Transactivation of target genes by GmWRI1s in Arabidopsis leaf protoplasts with renilla luciferase activity as reference. All data are from three biological replicates and expressed as means ± SD.