Figure 7.

Suppression of GmWRI1 expression affected nodulation and carbon partitioning in soybean nodules. The GmWRI1 knockdown hairy roots were generated by GmWRI1RNAi repression, and used for nodulation assay, metabolite and gene expression analysis. (a‐f) Characterization and metabolite analyses of GmWRI1RNAi knockdown hairy roots. qRT‐PCR confirmation of GmWRI1a and b expression (a), nodule numbers (b), the contents of TAG (c), DAG (d), soluble sugar (e) and starch (f) of GmWRI1RNAi hairy root lines compared with GFP line (as a control). (g) Expression of nodulation genes, glycolysis and lipid biosynthesis genes in GmWRI1RNAi hairy root lines compared with GFP line (as a control). Expression levels were expressed as means ± SD from three biological replicates. *P < 0.05 and **P < 0.01 by Student’s t‐test labelled for significant difference.