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. 2019 Dec 12;10:2884. doi: 10.3389/fmicb.2019.02884

TABLE 1.

Primers used for DPO-nanoPCR and conventional PCR.

Primer type Gene Name Primer sequences (5′→3′) Product size
Normal VP6 BRV-F TTTCCCTTATTCAGCTTCATTCACGTTGAACAGATCGCA 450 bp
BRV-R AACGCCGCTACCGCTGGTGTCATATTTGGTGGTCTCATC
DPO VP6 BRV-DPOF TTTCCCTTATTCAGCTTCATTCACIIIIIACAGATCGCA 450 bp
BRV-DPOR AACGCCGCTACCGCTGGTGTCATAIIIIITGGTCTCATC
Normal VP2 BPV-F AGCGAGAACATTGTGGTCACTAAAAACACTCGCCAGTTTA 325 bp
BPV-R AGATGTGCATGCCTGCAGTCAGATCATTGTTGTAGACGG
DPO VP2 BPV-DPOF AGCGAGAACATTGTGGTCACTAAAAIIIIICGCCAGTTTA 325 bp
BPV-DPOR AGATGTGCATGCCTGCAGTCAGATIIIIITTGTAGACGG
Normal 5′UTR BVDV-F GTTGGATGGCTGAAGCCCTGAGTACAGGGTAGTCGTCA 180 bp
BVDV-R TGCAGCACCCTATCAGGCTGTATTCGTAGCGGTTGGTTA
DPO 5′UTR BVDV-DPOF GTTGGATGGCTGAAGCCCTGAGTIIIIIGTAGTCGTCA 180 bp
BVDV-DPOR TGCAGCACCCTATCAGGCTGTATTIIIIICGGTTGGTTA